Raf kinase inhibitor protein , RKIP , catalyzes prasugrel as a bioactivation hydrolase

250 words Introduction: 315 words Discussion: 950 words Abbreviations: HPLC, high performance liquid chromatography; LC-MS/MS, liquid chromatography/tandem mass spectrometry; RKIP, Raf1-kinase inhibitor protein; HEPES, 2-[4-(2-Hydroxyethyl)-1-piperazinyl]ethanesulfonic acid; DMSO, dimethylsulfoxide ; Tris, tris(hydroxymethyl)aminomethane, SDS-PAGE, sodium dodecyl sulfate-polyacrylamide gel electrophoresis ; GFP, green fluorescent protein; prasugrel, 2-acetoxy-5-(α-cyclopropylcarbonyl-2-fluorobenzyl)-4,5,6,7-tetrahydrothieno[3,2-c]pyr idine; hCE, human carboxylesterase; thiolactone metabolite, 2-[2-oxo-6,7-dihydrothieno[3,2-c]pyridin-5(4H)-yl]-1-cyclopropyl-2-(2-fluorophenyl) ethanone; R-135766, (2Z)-(1-{2-[(2,2,3,3-H4)Cyclopropyl]-1-(2-fluorophenyl)-2-oxoethyl}-4-[( H3)methyls ulfanyl]piperidin-3-ylidene)ethanoic acid. This article has not been copyedited and formatted. The final version may differ from this version. DMD Fast Forward. Published on November 11, 2015 as DOI: 10.1124/dmd.115.066290 at A PE T Jornals on Jne 3, 2017 dm d.aspurnals.org D ow nladed from